Commuters observe a minute of silence at the Atocha railway station for victims of the high-speed train collision. (AFP pic)TOLEDO: Spain began demanding answers on Thursday after its second high-speed train collision this year killed 69 people and injured scores, with distraught relatives desperately seeking news about their loved ones.

One of Europe's deadliest rail disasters this century struck south of Madrid on Wednesday morning when a service by state operator Renfe ploughed into the back of a stationary train belonging to private Spanish company Iryo.

Manuel San Emeterio, whose 54-year-old brother Pepe is missing, came to the central city of Toledo to provide DNA samples to the Civil Guard.